Understanding PCR A Practical Bench-Top Guide by Sarah Maddocks
This Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to
- Includes resources for reagents
- Explains basic laboratory preparation
- Provides straightforward experimental protocols
- Incorporates fundamental analytical techniques
- Contains a troubleshooting guide
A user friendly book that features step-by-step basic protocols, helpful resource recommendations, and basic troubleshooting tips, this approachable and practical guide explains the process for setting up and analyzing one’s first PCR reaction.
So, you have decided to tackle the polymerase chain reaction (PCR); this is an extremely powerful technique that will enable you to amplify fragments of DNA. PCR is also incredibly versatile, and once you have mastered the basics, you will be able to apply the techniques you have learned to a host of molecular techniques including genetic proﬁling, detection, gene expression, and genetic modiﬁcation.
The world of PCR can be overwhelming, from deciding on your primer design, to where to buy your polymerase. It is therefore important that you understand the fundamental requirements necessary to prepare and carry out PCR, to avoid months of frustration or results you can not even begin to comprehend.
- Book Name: Understanding PCR A Practical Bench-Top Guide by Sarah Maddocks
- File Type: PDF
- Download: Link
- Size: 9 MB
- Password: PDFLibrary.Net